【摘要】 宫颈锥形切除术是宫颈上皮内病变诊断和治疗的重要方法，已广泛应用于临床，而部分患者锥切术后因特殊原因如宫颈浸润癌等需进一步切除子宫。研究显示，宫颈锥切术后可导致残留宫颈组织在短时间内出现炎症病变，从而导致周围组织粘连，进而增加后续子宫切除手术的操作难度及术后并发症，然而时间间隔过长可能病变进一步发展。关于子宫切除手术时机的选择，目前仍存在争议，本文就宫颈锥形切除术后行子宫切除术的时机作一综述。

慢性子宫内膜炎（chronic endometritis, CE） 属于盆腔炎性疾病的一种， 是子宫内膜的局部炎症，
慢性子宫内膜炎的间质部常常有大量浆细胞及淋巴细胞， 因为症状的非特异性在临床工作中常常被忽视。 近年
来， 许多研究发现， 慢性子宫内膜炎与不孕症、 许多产科和新生儿并发症有关， 故而逐渐引起临床医生重视。 现
就慢性子宫内膜炎的发病原因及相关预防措施作一综述。

【Abstract】 Objective To compare the pregnancy outcomes of luteal support protocols by methods of endometrium preparation in frozen-thawed embryo transfer cycles. Methods The retrospective study included the frozen-thawed embryo transfer cycles using vaginal micronized progesterone for luteal support in Sun Yat-sen Memorial Hospital of Sun Yat-sen University from January 2017 to December 2017, including 437 patients in the natural cycle group and 416 patients in the hormone replacement cycle group. According to different luteal support programs, they were divided into four groups: ① Group A: Progesterone soft capsule 400 mg/d; ② Group B: Progesterone soft capsule 400 mg/d+dydrogesterone 20 mg/d; ③ Group C: Progesterone soft capsule 600 mg/d; ④ Group D: Progesterone soft capsule 600 mg/ D+dydrogesterone 20 mg/ D. Different endometrial preparation regimens, luteal support dosages and pregnancy outcomes were compared. Results ①There was no significant difference in the clinical pregnancy rate, abortion rate and live birth rate among different luteal support schemes in the natural cycle group (P>0.05).② The clinical pregnancy rate in group C was lower than that in group D (35.50%, 51.30%, P=0.008). ③ In group C of hormone replacement cycle, the miscarriage rate of patients <35 years old was lower than those ≥35 years old (7.14%, 36.80%, P=0.004). In group C, the clinical pregnancy rate of patients <35 years of age in the hormone replacement cycle group was lower than that in the natural cycle group (38.90%, 68.30%, P=0.002). Conclusion Enhancing luteal support in artificial cycles would improve the pregnancy outcomes of frozen-thawed cycles.

【摘要】 子宫内膜透明细胞癌（endometrial clear cell carcinoma， ECCC）具体发病的分子机制不明。依据癌症基因组图谱（The Cancer Genome Atlas, TCGA）子宫内膜癌分子分型检测流程结果显示，ECCC中TP53突变率远低于浆液性癌，微卫星不稳定性和POLE基因突变的检出率罕见。p53蛋白异常表达可能作为ECCC临床管理和预后评估的重要参考指标，而POLE突变和MSI状态可能为具有透明细胞特征或透明细胞癌成分的混合性子宫内膜癌提供克隆性起源、分化方向和治疗预测的分子依据。

Objective To investigate the effect of gonadotropin-releasing hormone agonist (GnRH-a)
combined with low-dose human chorionic gonadotrophin (hCG) on the clinical outcome of in-vitro fertilization-embryo
transfer (IVF-ET) in patients with high ovarian response. Methods A retrospective analysis was performed on the clinical
data of 546 patients who underwent controlled superovulation stimulation via (IVF/ICSI) in our hospital from December
2016 to December 2017. Patients adopting GnRH-a + 5 000 IU hCG dual-trigger (hCG high dose group, n=159) or
GnRH-a + 2 000 IU hCG dual-trigger (hCG low dose group, n=212) were enrolled. Those using the standard hCG 10 000 IU
trigger were involved as a control group . The treatment outcome of each group was compared. Results There was no
significant differences in basic dosage or medication time of gonadotropin trigger among the three groups (P >0.05). The
serum levels of estradiol (E2) on ovulation induction day in the high-dose hCG group [(17 513.52±4 846.85) pmol/L]
and the low-dose hCG group [(20 384.11±7 754.78) pmol/L] were significantly higher than those in the control
group [(16 678.67±3 826.79) pmol/L] (P=0.000). The number of retrieved oocytes, number of 2PN embryos, number
of embryos, number of high-quality embryos, and rate of high-quality embryos in both test groups were all significantly
doi:10.13390/j.issn.1672-1861.2020.02.006
基金项目： 国家自然科学基金项（81471457）； 南京医科大学科技发展基金面上项目（2017NJMU075）
作者单位： 210000 南京医科大学附属妇产医院， 南京市妇幼保健院生殖医学中心
通信作者： 张娟 Email： sallylee_87@126.com
● 134 ● 中国妇产科临床杂志 2020 年 3 月 第 21 卷 第 2 期 Chin J Clin Obstet Gynecol March 2020， Vol.21， No.2
higher than incontrol group (P <0.05). The rate of high-quality embryos in hCG high dose group was significantly larger
than in hCG low dose group. There were no statistically significant differences in clinical pregnancy rates (53.7%, 50.0%,
51.9%), ectopic pregnancy rates (10.3%, 14.3%, 0%) and abortion rates (10.3%, 0%, 7.1%) among the three groups,
Tnumber of ovarian hyperstimulation syndrome (OHSS)and the incidence of OHSS rate (2.9%, 1.3%, 0.5%) were not
significantly different (P >0.05). Conclusion For patients with high response to GnRH antagonist regimen, the use of
low-dosage dual-trigger can increase the rate of high-quality embryos and the number of transferrable embryos without
increasing the incidence of OHSSs.

目的 研究单脐动脉对妊娠期并发症及新生儿结局的影响。 方法 对 171 例本院分娩的单脐动脉
病例进行回顾性分析， 比较同期胎儿非单脐动脉孕妇（1 123 例） 及足月分娩的单脐动脉新生儿（84 例） 与同期
分娩的非单脐动脉新生儿（336 例） 的母婴结局。 结果 单脐动脉孕妇并发双胎妊娠为 8.2%（14/171）， 子痫前
期为 9.4%（16/171）， 合并子宫发育异常为 9.4%（4/171）， 三者均较对照组高（P ＜ 0.05）； 单脐动脉组新生儿出
生体质量 [（3 224±379） g] 与对照组 [（3 334±426） g] 比较， 差异有统计学意义（P ＜ 0.05）， 但新生儿重症监
护病房（NICU） 转诊率（9.5%， 8/84）， 新生儿窒息率（0， 0/84）， 羊水污染率（9.5%， 8/84） 比较， 差异均无
统计学意义（P ＞0.05）。 结论 胎儿单脐动脉孕妇并发症增加， 尤其是子痫前期发生增加， 新生儿出生体质量偏
低， 应加强监护。

慢性子宫内膜炎（chronic endometritis, CE） 临床特点多样， 无特异性诊断方法， 在临床实践中易
被漏诊。 近年来的研究表明， CE 与不明原因不孕、 反复种植失败和复发性流产等不良生殖疾病密切相关。 CE 主
要由各种病原体感染引起的子宫内膜炎症， 因此， 明确易于导致子宫内膜感染的微生物及其致病性， 将有助于改
善 CE 的诊治现状， 而微生物组学为此提供了更大的可能性。 本文将对近年来子宫内膜微生物组学及微生物组学
在 CE 诊治中的应用研究进展进行综述。

【摘要】 目的 探讨不同宫颈鳞状上皮内病变患者的p16及ki-67蛋白的表达及其意义。方法 收集宫颈活检和宫颈锥切术资料完整的宫颈鳞状上皮内病变（SIL）患者92例作为研究组，其中26例宫颈上皮内瘤变（CIN）1，23例CIN2，43例CIN3，选择同期宫颈炎症25 例作为对照组。采用免疫组织化学法检测宫颈组织中p16蛋白及ki-67蛋白的表达情况并分析。结果 （1） 在不同CIN组织中，p16和ki-67蛋白阳性表达率明显不同（P<0.001），p16阳性表达率随着CIN严重程度的增加而升高（P<0.05）；（2） 在不同CIN组织中，p16和ki-67蛋白共同阳性率明显不同（P<0.001）。p16和ki-67蛋白阳性表达率随着CIN严重程度的增加而升高（P<0.05）。结论 p16和ki-67蛋白可以区别不同程度CIN，加强两者的检测可辅助CIN2/3的诊断及预后的判断。

【Abstract】 Objective To study the differences of drug sensitivity of buconazole, fluconazole, itraconazole, miconazole and clotrizole to drug-resistant candida strains. Methods The minimum inhibitory concentration (MIC) of five azole drugs against 120 strains of drug-resistant candida albicans and 8 strains of non-candida albicans were measured and compared according to the microliquid-based dilution method of M27-A3 regimen recommended by the American Association for Clinical Laboratory Standardization (CLSI). Results ①The 24 h MIC of buconazole was lower than that of other four kinds of antifungal drugs.② The MIC value of itraconazole against candida albicans niketriazole (1.82 μg/ml) was higher than the defined value of resistance (1 μg/ml).③ In the fluconazole resistant group, miconazole resistant group and itraconazole resistant group, the MIC values of cloxazole (0.56 μg/ml, 0.39 μg/ml and 0.52 μg/ml) were far lower than the sensitive value (2 μg/ml), and the MIC values of itraconazole (2.44 μg/ml, 2.11 μg/ml and 2.23 μg/ml) were far higher than the resistant value (1 μg/ml). Conclusions The drug sensitivity of buconazole to drug resistant candida is better than the other four azole drugs.

Objective To detect the values of serum tumor markers including CA₁₂₅,CA199,AFP and CEA and to assess their value in the diagnosis of mature cystic teratoma (MCT). Methods Data of 512 patients with cystic teratoma of ovary diagnosed by postoperative pathology who treated in our hospital were analyzed retrospectively. Results 492 cases were tested for serum CA₁₂₅. Among these 492 patients, 44 had elevated CA₁₂₅ levels (>35U/ml). If we excluded the 24 cases who suffered from other diseases which could cause increased CA₁₂₅ levels, the positive rate was 4.27%. 101 patients were tested for serum CA199 and the mean level was 47.10 U/ml. 41 had the CA199 levels above 37 U/ml with the positive rate of 40.59%.149 patiens were detected AFP and no abnormal AFP was found. 93 cases were tested for CEA.Only one was abnormal and the positive rate was 1.08%. The mean diameter of the CA199 positive group ((8.32±3.19)cm) was bigger than that of the negative group ((6.43±3.12)cm,P< 0.05)).But the elevated level of CA199 had no correlation with tumor location, age,and nerve tissue in the tumor. Conclusions CA199 is the only tumor marker which has clinical significance and the highest positive rate for MCT. The diameter of the tumor with positive CA199 may be bigger than those with negative CA199.

Objective To evaluate the value of hysteroscopy in the diagnosis of chronic endometritis (CE).
Methods A retrospective analysis was performed from September 2016 to December 2017, 1320 women were
admitted to Hysteroscopy centre of FuXing Hospital, Capital Medical University All of them underwent hysteroscopy
and endometrial biopsy for histological analysis. Sensitivity, specificity and accuracy of the hysteroscopic features in
the diagnosis of CE were analyzed. Result Among 1 320 patients, endometrial immunohistochemistry CD138 was
positive in 345 cases (26.1%) as the histopathological diagnostic criteria for CE. Hysteroscopy showed that endometrial
hyperemia accounted for 53.0% (183/345), endometrial interstitial edema for 8.4% (29/345), and endometrial micropolyps
for 3.2% (11/345). The kappa value of intra-observer and inter-observer agreement of the presence or absence of the
hysteroscopic feature of CE was 0.86 and 0.73 respectively. One or more CE features under hysteroscopy were used as
the diagnostic criteria for CE. The sensitivity, specificity, positive and negative predictive value and diagnostic accuracy
of CE were 60.3% (208/345), 69.6% (679/975), 41.3% (208/504), 83.2% (679/816) and 67.2% (887/1320) respectively.
Conclusion The finding of endometrial hyperemia, micro-polyps or endometrial interstitial edema during hysteroscopy
should be alert to the presence of CE. However, since the accuracy of diagnosing CE by hysteroscopy is still low, it cannot
replace histophiological examination.

目的 探讨卵巢成熟畸胎瘤癌变的临床病理特征。 方法 回顾性分析 7 例卵巢成熟畸胎瘤癌变患
者的临床组织病理学资料。 结果 2012 年 9 月至 2018 年 12 月本院收治卵巢成熟畸胎瘤 599 例， 其中成熟畸胎
瘤恶变 7 例（1.17%）。 7 例癌变患者中， 类癌 4 例， 卵巢、 鳞状细胞癌、 甲状腺肿伴类癌和甲状腺乳头状癌各 1
例。 7 例均表现为盆腔包块， 其中 1 例盆腔包块 28 年； 伴异常子宫出血 2 例； 伴排尿困难 2 例； 伴下腹痛 2 例。
1 例类癌 28 年前体检发现肿物， 随访逐渐增大， 手术治疗； 1 例类癌月经紊乱 1 年， 阴道流血 3 个月并增多要求
手术； 其余 5 例体检发现肿物， 药物治疗效果不佳， 1 周 ~1 年内手术治疗。 1 例鳞癌及甲状腺乳头状癌患者行手
术切除， 术后辅助化疗； 其余 5 例患者单纯手术切除。 随访 6 个月 ~6 年， 均无复发和转移。 结论 卵巢成熟畸
胎瘤癌变概率非常低， 但预后较好 , 有畸胎瘤病史， 年龄大者， 尤其绝经后发现盆腔包块， 应高度警惕癌变， 建
议尽早手术。

Objective To study the prevalence of chronic endometritis (CE) in patients with recurrent
implantation failure (RIF) and whether the pregnancy outcomes were improved after CE treatment. Methods The
clinical data of 287 patients who underwent hysteroscopy and endometrial pathology for RIF from January 2016 to
January 2017 in the Reproductive Medical Center of The Peking University Third Hospital were retrospectively analyzed.
The prevalence of CE in RIF patients and the accuracy of hysteroscopy diagnosis of CE were analyzed. The patients
were divided into RIF-CE group (n=111) and RIF-NCE group (n=176 cases) according to whether there was concomitant
CE.The RIF-CE group were treated with antibiotics for 2 weeks, and the RIF-NCE group received no other intervention
except hysteroscopy and endometrial biopsy. The pregnancy outcomes of the first thawing cycle transplantation were
compared between the two groups. Results The prevalence of CE in RIF patients was 38.7% (111/287). The sensitivity
and specificity of hysteroscopy in diagnosing CE were 55.0% (95% CI:46.1~64.3%) and 82.4% (95% CI:77.2~88.1%).
After RIF-CE treatment, the implantation rate (50.0% vs 36.4%) and the clinical pregnancy rate (53.8% vs 39.3%) of
blastocyst transfer in thawing cycle were significantly higher than those in RIE-NCE group (P <0.05). Conclusion It is
suggested that RIF patients should be screened for CE by hysteroscopy, and the pregnancy outcome of RIF patients can be
improved by embryo transfer after active treatment of CE.

Objective To investigate the expression of E-cadherin, Snail and N-cadherin and their relationship in cervical carcinoma. Methods The expression of E-cadherin, Snail and N-cadherin in tissues from 42 patients with chronic cervicitis, 29 cervical intraepithelial neoplasia(CIN group)and 44 cervical carcinoma (CA group) were detected by immunohistochemistry (SP method). Results Compared with chronic cervicitis, the expression of E-cadherin was significant decreased, the Snail and N-cadherin were significant increased in CIN and CA group (P<0.05). The expression of N-adherin was correlated positively with Snail (r=0.671,P<0.01); there was a significant negative correlation between expression of E-cadherin and N-adherin (r= -0.643,P<0.01),as well as between expression of E-cadherin and Snail (r=-0.492,P<0.01). Conclusions The lower E-adherin expression and higher level of Snail and N-cadherin may be play some role on the pathogenesis of cervical cancer.

【摘要】 目的 探讨精子DNA碎片指数（DFI）对体外受精-胚胎移植（IVF-ET）临床结局的影响。方法 选自2017年1月至2019年2月在本院生殖中心行IVF-ET新鲜及解冻周期移植的不孕症患者1 885例，通过检测男方精液常规与DFI，比较精液参数、不同年龄、不同受精方式等临床资料，观察其临床结局。结果 ① 精液参数中，DFI>30组 精子密度、（a+b）活力、畸形率、流产率与其他两组≤15/15~30比较有统计学意义（P<0.05）；② 年龄≥35岁组DFI值、流产率、临床妊娠率，与其他两组（21~29）岁、（30~34）岁比较有统计学意义（P<0.05）；③ ICSI组DFI值、流产率，与其他两组IVF/RICSI比较，差异有统计学意义（P<0.05），但三组间临床妊娠率比较，差异无统计学意义P>0.05。结论 DFI>30能引起精子密度、（a+b）活力下降，精子畸形率增高，虽不显著影响临床妊娠率，却会增加流产率。

【摘要】 目的 探讨胎儿病理性小肠扩张的超声预测指标，为产前咨询提供指导。方法 将2013年1月至2020年6月在北京大学第一医院行胎儿超声检查、结果提示胎儿小肠扩张并有完整随访结局的52例单胎孕妇纳入研究，根据新生儿是否存在肠道异常将患者分为肠道异常组和肠道正常组，比较两组间临床资料和产前超声征象的差异，并利用ROC曲线分析肠管宽度对胎儿病理性小肠扩张的预测价值。结果 52例新生儿中，19例新生儿存在异常（其中小肠闭锁13例、小肠扭转3例、空肠隔膜2例、肠套叠1例）。单因素分析显示，肠道异常组的产前小肠宽度显著大于肠道正常组，差异有统计学意义（P=0.000）。通过 ROC曲线分析显示，以16.7 mm作为判断小肠扩张预后的临界值时，其曲线下面积(AUC)为0.914，敏感度、特异度、阳性预测值、阴性预测值和准确率分别为84.2%、93.9%、88.9%、91.2%和90.4%。结论 胎儿小肠宽度大于16.7 mm对病理性小肠扩张的预测价值较高。

【摘要】 目的 探讨点阵式CO2激光治疗外阴阴道萎缩（vulvovaginal atrophy, VVA）的临床疗效。方法 招募2017年5月至2019年5月北京大学深圳医院妇科门诊就诊的VVA患者，使用点阵式CO2激光治疗仪进行阴道内治疗3次，每次间隔4~6周。采用阴道健康指数评分（vaginal health index score, VHIS）及视觉模拟评分法（visual analogue scale, VAS）评估治疗前后VVA症状和疼痛程度，并进行满意度调查。结果 治疗前VHIS结果（9.77±1.50）分，第一次治疗后VHIS（11.53±1.76）分，在1、3和6个月的随访结果分别是（14.43±1.43）分、（15.80±1.35）分和（16.33±1.21）分，治疗前后比较，差异均有统计学意义（P＜0.05）。VVA症状评分显著改善[阴道灼痛：（64.67±10.74）分和（48.00±9.97）分；阴道干涩：（62.67±10.48）分和（45.33±5.07）分；阴道瘙痒：（63.00±10.55）分和（47.67±9.71）分；性交痛：（66.00±11.02）分和（55.00±9.74）分]治疗前后比较，差异有统计学意义（P<0.05）。30例患者在激光探头插入、移动和激光治疗时疼痛轻微，治疗总体满意率90%（27/30），无不良事件发生。结论 点阵式CO2激光治疗能够显著改善绝经后女性VVA的症状，明显提高患者VHIS，改善患者的生活质量和身心健康。

【Abstract】 Objective To evaluate the efficacy and safety of Fuyanshu capsule(FC) combined with antimicrobial agents in the treatment of dampness-heat stasis syndrome of pelvic inflammatory disease (PID). Methods A prospective, multicenter, randomized, double-blind design was adopted. A total of 288 PID patients aged 20 to 50 years were enrolled in 5 hospitals from June 2017 to September 2018, and randomly assigned to study group (treated with Fuyanshu capsule) and control group (treated with placebo) according to a 1:1 ratio. 144 cases in both groups were additively given levofloxacin tablets + metronidazole tablets from the 1st to 14th day of admission. The score of McCormack scale and traditional Chinese medicine (TCM) syndromes were compared between the two groups after 8 weeks of treatment (after 2 menstrual cycles). Follow-up was performed one month after drug withdrawal. Results The score of McCormack scale in the study group was lower than that before treatment [(7.65±2.21) points] and higher than that in the control group [(4.01±2.46) points] (P<0.0001). The effective rate of TCM syndrome in the study group [93.75% (135/144)] was higher than that in the control group [41.67% (60/144)] (P<0.0001).The recurrence rate of the study group was 3.50% (5/144) and the incidence of pelvic pain was 10.49% (15/144) within one month after drug withdrawal, both of which were lower than those of the control group (P<0.0001). Conclusions Antibiotics combined with FC improved the score of McCormack scale, curative effect of TCM syndromes, disease recurrence and sequelae of pelvic pain in PID patients better than antibiotics alone.

Objective To understand the role of single-time colposcopy and HPV-DNA genotyping test in the diagnosis of HSIL patients. Methods 551 patients whose cervical cytology test results were HSIL underwent immediate colposcopy detection from January 2005 to December 2007,multiple punch biopsy was taken for 519 patients who had abnormal findings under colposcopy, for those unsatisfied colposcopy test, cervical curettage were added . Results Among the 551 cases of HSIL patients,there were 58 (10.5%) cases of CIN 1, 334 (62.4%) cases of CIN 2 and CIN 3, 11 (2%) cases of cervical cancer diagnosed by multi-point biopsy under single-time colpscopy. The occurrence in different age groups had significant difference (P=0.034). Meanwhile 376 HSIL patients received HPV-DNA genotyping test, the positive rate of high-risk HPV was 87.5%, and the most common subtype was HPV 16 ( 45.7%). In HR -HPV positive group, especially HPV 16 subtype positive group, the detection rate of CIN2 was significant higher than that in the HR-HPV negative group (P=0.000). Conclusions Only 62.4% patients of CIN2 and more advanced lesion were diagnosed under single-time colposcopy. HPV-DNA genotyping test may help increasing the detection rate of CIN and cervical cancer.We should pay more attention to HSIL patients whose colposcopy test has no abnormal findings.

【Abstract】 Objective To explore the appropriate surgery by analyzing the pathology and prognosis of patients with high-grade squamous intraepithelial lesion (HSIL) before and after surgery. Methods A total of 528 patients with cervical HSIL diagnosed by colposcopic biopsy were treated with cold knife conization (CKC) (n=353) and loop electrosurgical excision procedure (LEEP) (n=175) respectively, whose pathological results were residual HSIL and cervical cancer need reoperation. The distribution of pathological grade before and after operation was compared, reoperation, positive margin rate, residual rate, recurrence rate and the HPV negative conversion rate and the persistent positive rate within one year were analyzed. Results In the diagnosis of cervical HSIL, the coincidence rate between CKC and biopsy pathology was 72.52% (256/353) (P<0.0001). The coincidence rate between LEEP and biopsy pathology was 58.86% (103/175) (P<0.0001). The positive margin rate after CKC was 7.41% (20/270), and the residual and recurrence rate was 5.93% (16/270). The positive margin rate after LEEP was 5.66% (6/106), and the residual and recurrence rate was 4.71% (5/106). The HPV negative conversion rate was 69.80% (104/149) and 56.06% (37/66) within one year after CKC and LEEP, respectively, and the persistent infection rates of high-risk HPV were all decreased. Conclusion CKC and LEEP are consistent in the diagnosis of cervical HSIL, and the negative conversion rate of HPV was high within one year follow-up, but individualized surgical strategy still should be developed according to multi-factor comprehensive analysis.

Objective To assess the relationship between increment of nuchalskinfold thickness and chromosomally abnormal fetuses by ultrasonography. Methods Nuchalkinfold thickness(NT) of pregnant women(10~22 weeks) collected by prenatal ultrasonic B in in the People's Hospital Peking University from Feb.2007 to Dec. 2009.Those with increment of NT were suggestted to perform amniocentesis and cordocentesis chromosomal karyotypes analysis and were under followed-up. Results There were totally 78 cases with increment of NT,3 cases were chromosomally abnormal and all of the 3 cases were Down syndrome. The incidence of Down syndrome was 3.85% these were tatally.There were 6 cases with bad pregnant results,incluing 5 cases with abnomal fetuses which included right hydrencephaly and liquorrhea,polycystic kidney,teratic heart,rubella virus infection and hydrosarca. Conclusions Increment of NT measured by ultrasound may play an important guiding role in diagnosising Down syndrome.It is one of the main markers at early and middle stage pregnancy.

Objective To discuss the role of age and HR-HPV in evaluation of cervical lesions with negative Liquid-Based Cytology. Methods 906 women with positive HPV DNA and negative cytology results were included. The sampling methods were standardized. All cases underwent colposcopic indicated biopsy and pathological diagnosis. Analysis were based on the final results. Results The average age was (36.69±9.06 )years old. Pathological results showed 3 invasive cancer (2 micro-invasive cancer included), 1 adenocarcinoma, 29 cases of CIN3, 50 of CIN2, 155 of CIN1,2 of VAIN1 and 666 of normal. The odds ratio of ≥CIN3 between 30~49 years and <30 years was 3.4(1.02~11.40) while HR-HPV DNA load between ≥100 and <10 was 12.44 (2.89~53.5). Prevalence rate of ≥CIN3 in low, median, high HR-HPV load group 30~49 age group was 1.0%,4.0%,8.8%(χ² =14.978,P=0.001) while the prevalence rate of ≥CIN2 was 4.3%,7.5% and 19.1%(χ² =27.270,P=0.000)respectively. The average HR-HPV load of non-CIN patients in <30 years group and ≥30 years group were 244.04±547.20 and 149.59±328.39 (P<0.050). Conclusions Increased HR-HPV load are generally associated with an increasing risk of high-grade CIN. Women who are in the high risk age group should be assessed by HR-HPV load and be performed colposcopy to minimize the missed diagnostic cases of high grade lesions.

Objective To investigate the clinical application value of surface plasmon resonance( SPR) in detecting human papillomavirus subtype in patients with cervical lesions. Methods 109 patients who underwent screening for cervical lesions in Peking University People's Hospital and had not received any cervical treatment before were enrolled. Cytology was performed for everyone by liquid- based Thin- prep test. Colposcopy and biopsy were performed on patients who had a cytology result of ASCUS or greater. Testing for high- risk human papillomavirus (HPV) DNA were performed using HC2 method and HPV subtypes were detected using SPR method. These results were compared with the cloning and sequencing results. TERC gene expression were detected by FISH. Results (1) Regarding cloning and sequencing results as gold standard, the specificity of HPV-SPR in detecting high- risk HPV was significantly higher than HC2 (P<0.05), and there was no significant difference in sensitivity between them (P>0.05). (2) In term of the pathology results, the sensitivity and NPV of SPR in identificating CIN 2 and above lesions were significantly lower than that of HC2 method in distinguishing the CIN 2 and above lesions ( P<0.05). There was no significant difference in specificity and PPV between the two methods (all P>0.05). The specificity of TERC detecting in distinguishing the CIN 2 and above lesions was significantly higher than that of HC2 method (P<0.01). There was no significant difference in sensitivity, PPV and NPV between this two methods (P>0.05). Conclusions The specificity of SPR method in detecting high- risk HPV was higher than that of HC2 method. The sensitivity of the two methods in detecting high- risk HPV was comparative. Utilizing SPR method in high lesions can improve the specificity of liquid- based cytology and HC2 test and could avoid unnecessary colposcopy and treatment.

Objective To analysis the diagnosis, treatment and outcome of malignant tumor in pregnancy, in order to improve the outcome of mother and fetal. Methods Data of 9 pregnant patients with malignant tumor who were treated in Shanghai Jiaotong University No. 6 People's Hospital from 2007 to 2010 were reviewed. The patients include 2 lung cancer, 2 gastric carcinoma, 1 hepatocellular carcinoma,1 Nasopharyngeal carcinoma,1 brain neurogliocytoma,1 breast adenocarcinoma and 1 ovarian cancer. Results Of the 9 patients, only 4 had regular prenatal examination; 2 patients were diagnosed before pregnancy,the other 7 were diagnosed during pregnancy;7 patients were diagnosed by pathology and 2 patients were diagnosed by clinical imageology. 7 patients were in advanced stage, the other 2 were in early stage.Pregnant outcomes included 1 early induced abortion, 2 middle induced abortion, 2 vaginal delievery and 4 cesarean section delievery. As for the fetus outcome, there were 5 preterm infant and 2 term infant;6 newborn infants had good outcome,1 preterm fetal dead in uterus; 5 patients received treatment after delivery, other 4 didn't received any treatment after delivery;6 patients died during the first year's follow-up. Conclusions The deficient of the recognition of malignant tumor, lack of full-scale perinatal monitoring, short of perfect analyses of history, symptoms and sign of malignant disease and the delayed treatment for malignant tumor being considering the adverse effect on fetal were the main causes of the delay diagnosis and treatment of malignant disease in pregnancy. The pregnancy with malignant tumor was associated with poor maternal outcome but no unnormal fetal development was found.

Objective To study the efficacy of Levonorgestrel-releasing intrauterine system (LNG-IUS Band name: Mirena) on the treatment of adenomyosis. Methods 65 patients with adenomyosis who received LNG-IUS placement recruited in this study. The visual analogue seale (VAS) scores, menstrual blood volume and uterine volume were compared before and after 6 and 12 months LNG-IUS placement. Results Compared with before treatment,dysmenorrhea was completely relieved or significantly alleviated in all patients after 6 month treatment of LNG-IUS. The mean VAS scores dropped from 81.3±9.4 to 16.8±12.1(P<0.01), the menstrual blood volume reduced significantly [100% vs (25.3±12.2)%, P<0.01]. After 12 months, hemoglobin recovered to normal in 30 patients with anemia (P<0.01). Uterine volume reduced and had significantly difference compared to before treatment (P<0.05). Conclusions LNG-IUS is an efficient method to treat adenomyosis, it can alleviate dysmenorrheal, decrease menstrual blood volume and reduce uterus volume.

Objective To construct cDNA of laminin receptor and study it's protein expression. Methods The cDNA of laminin receptor was amplified from total RNA of HeLa cells and was ligated into pGEM-T Easy plasmid. Recombinant LRP was expressed by using pET 22b(+).coli expression system. Results Restriction endonucleases mapping analysis and DNA sequencing demonstrated that the constructed plasmid was the recombinant pET-LRP. A new band of protein was found in SDS-PAGE. Conclusions The construction of pET-LRP is succeed . This will be a basis of further study on the structure and biological function of LRP.