15 July 2025, Volume 26 Issue 4
    

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  • Diagnosis, treatment and related issues of PARP inhibitor resistance in ovarian cancer#br#
    Chinese Journal of Clinical Obstetrics and Gynecology. 2025, 26(4): 289. https://doi.org/10.13390/j.issn.1672-1861.2025.04.001
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  • Synthesis of MRI/near-infrared fluorescence dual-modality probe and in vivo imaging study in animal model of ovarian cancer
    ZHANG Yijun, ZHU Chongyuan, LI Changjian, ZHANG Chen, CHANG Xiaohong, LI Yi
    Chinese Journal of Clinical Obstetrics and Gynecology. 2025, 26(4): 292-296. https://doi.org/10.13390/j.issn.1672-1861.2025.04.002
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    Objective To synthesize a MRI/near-infrared (NIR) fluorescence dual-modal molecular probe and evaluate its specificity in targeting ovarian cancer in vivo. Methods The MRI/NIR dual-modal probe USPIOs-COC183B2-IRDye800CW was prepared. The hydrodynamic size and zeta potential of ultrasmall superparamagnetic iron oxides and dual-modal probes were measured. The subcutaneous xenografted tumor model of ovarian cancer was established. At different time points post-injection, the nude mice were scanned using MRI and NIR imaging systems to study the distribution and targeting of the probe. Vital organs and tumor tissues were isolated for ex-vivo fluorescence imaging and pathology validation. Results The average sizes of USPIOs and bimodal probe under TEM were (3.5±0.3) nm and (3.7±0.4) nm, respectively; Zeta potentials of (10.2±0.7) mV, (-10.3±0.8) mV, respectively. Both MRI and NIR imaging showed that the dual-modal probe could specifically aggregated in tumor. The probes were mainly distributed in liver and tumor sites of nude mice in the test group. Prussian blue staining indicated iron deposits in tumor of the test group. Conclusions The dual-modal molecular probe USPIOs-COC183B2-IRDye800CW can accurately indicate ovarian cancer lesions in vivo both MR and NIR imaging.
  • Risk assessment of hiding HSIL in people over 50 years old who have been LSIL for 2 years or more
    MI Lan, LI Jiayue, WANG Xiaojun, ZHANG Dai, BI Hui.
    Chinese Journal of Clinical Obstetrics and Gynecology. 2025, 26(4): 297-301. https://doi.org/10.13390/j.issn.1672-1861.2025.04.003
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    Objective To explore the risk of hiding HSIL in patients over 50 years old who have been suffering from LSIL for 2 years or more and the necessity of diagnostic cervical conization. Methods From January 2013 to October 2023, the clinical data of 136 women with LSIL who were over 50 years old and lasted for 2 years or more in Peking University First Hospital were retrospectively summarized, and the risk of hiding HSIL and the necessity of diagnostic cervical conization were evaluated. Results Among the 136 cases diagnosed as LSIL by colposcopy biopsy for 2 years or more, 47 cases (34.56%, 47/136) were diagnosed as HSIL after diagnostic cervical conization. There were 16 cases of HSIL (CIN 2) (11.76%, 16/136) and 31 cases of HSIL (CIN 3) (22.79%, 31/136). Pathology upgraded to cancer in 0 cases. Univariate analysis showed that the detection of pathological HSIL (CIN2+) and above after cervical conization was related to HPV positive (P=0.002), and logistic regression analysis showed that the OR value of HPV positive was 11.571 (95%CI: 1.491-89.724). Univariate analysis showed that pathological HSIL(CIN3) after cervical conization was related to age (P=0.045) and high-grade cytological abnormality (P=0.018). Logistic regression analysis showed that the OR value of high-grade cytological abnormality was 2.828 (95%CI: 1.237-6.469). Conclusions The risk of hiding HSIL is moderate in women over 50 years old with LSIL lasting for 2 years or more. Diagnostic cervical conization is recommended for those with high risk factors, such as HPV positive or high-grade cytological abnormalities.
  • The role of cervical cancer screening history in assessing the risk of cervical precancerous lesions#br#
    ZHANG Xiaosong, ZHU Xinxin, BI Hui, ZHAO Qingping, FU Yunfeng, WU Qiongyan, ZHANG Li'na, ZHANG Chunmei, HUANG Ning.
    Chinese Journal of Clinical Obstetrics and Gynecology. 2025, 26(4): 302-305. https://doi.org/10.13390/j.issn.1672-1861.2025.04.004
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    Objective To investigate the role of cervical cancer screening history in assessing the risk of cervical precancerous lesions/cervical cancer, and to provide evidence for personalized management of cervical lesions. Methods A total of 2 832 non-pregnant women who underwent colposcopy from September to December in 2021 from 6 hospitals were retrospective analysis. Results The risk of CIN2+ of women without cervical cancer screening history in recent 5 years was 72.2% (95% CI: 62.8%~80.4%) with HPV positive and cytology≥ASC-H, higher than 59.6% (95% CI: 45.8%~72.4%) of those with cervical cancer screening history; the risk of CIN3+ also reached 47.2% (95% CI: 37.5%~57.1%) , higher than 31.6% (95% CI: 19.9%~45.2%) of those with cervical cancer screening history in recent 5 years. The risk of CIN2+ of women without cervical cancer screening history was 58.3% (95% CI: 43.2%~72.4%) with cytology ≥ASC-H, higher than 45.5% (95% CI: 24.4%~67.8%) for those with cervical cancer screening history in recent 5 years, and 47.9% (95% CI: 33.3%~62.8%) for CIN3+, higher than the 19.0% (95% CI: 5.4%~41.9%) of women with screening history. Conclusions Regular cervical cancer screening can reduce the risk of cervical precancerous lesions/cervical cancer. It is suggested that the doctor should learn the patients about their previous cervical cancer screening history before colposcopy examination.
  • The clinical application value of molecular typing of endometrial cancer in the elderly
    ZHANG Xiaoli, ZHANG Xiaoxiao, LI Jialu, LI Weiping.
    Chinese Journal of Clinical Obstetrics and Gynecology. 2025, 26(4): 306-309. https://doi.org/10.13390/j.issn.1672-1861.2025.04.005
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    Objective To explore the molecular typing of elderly endometrial cancer and its clinical significance, and to provide a basis for individualized treatment. Methods A retrospective analysis was conducted on the general conditions, molecular typing, pathological features, surgical methods, and adjuvant therapy of 54 patients with endometrial cancer admitted to the Department of Obstetrics and Gynecology of the Third Center of the PLA General Hospital from May 2021 to August 2024. The oncological outcomes of the patients were followed up. Results The average age of the patients was (68±5.59) years, the average BMI was (26.6±3.92) kg/m2, and the average age of menopause was (54±3.63) years. There was a significant difference in the distribution of the p53abn subtype among different age groups (P=0.039). There were no significant differences in FIGO stage, pathological type, pathological grade, myometrial invasion, LVSI, risk factors, surgical methods, and adjuvant therapy among different age groups (P>0.05). The median follow-up time was 21.2 months (3 to 60 months), and there was no significant difference in overall survival among different age groups (P>0.05). Conclusions The introduction of molecular typing provides a new direction for individualized treatment decisions for elderly endometrial cancer, and emphasizes the survival concern for elderly patients, especially those over 70 years old.
  • The impact of endometrial thickness at different ages on pregnancy outcomes during fresh embryo transfer cycles in short-acting GnRH agonist long protocol
    LIU Chao, TAN Li.
    Chinese Journal of Clinical Obstetrics and Gynecology. 2025, 26(4): 310-314. https://doi.org/10.13390/j.issn.1672-1861.2025.04.006
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    Objective To investigate the impact of endometrial thickness (EMT) across varying age groups on pregnancy outcomes during fresh embryo transfer cycles in short-acting GnRH agonist long protocol. Methods A retrospective analysis was conducted of 3002 fresh embryo transfer cycles between June 2015 and May 2023 in the Department of Reproductive Medicine of the Second Affiliated Hospital of Zhengzhou University. The patients were classified into three groups: 25~29 years old, 30~34 years old and ≥35 years old. At the same time, according to the EMT on the day of human chorionic gonadotropin (hCG) administration, patients of all age groups were divided into four subgroups:<9 mm, 9~10.9 mm, 11~12.9 mm and ≥13 mm. The general data and pregnancy outcomes of each subgroup of patients in different age groups were compared. Results When the EMT was<9 mm, the rates of clinical pregnancy and live birth of each age group were significantly lower (P<0.05), and the trend was upward as the EMT increased. Multivariate logistic regression analysis showed that EMT on the day of hCG administration was an independent influencing factor for clinical pregnancy (P<0.001) and live birth (P<0.001). The optimal clinical pregnancy rates (OR: 1.980, 95%CI: 1.352-2.898, P<0.001) and live birth rates (OR: 2.387, 95%CI: 1.611-3.536, P<0.001) can be achieved when EMT ranged from 11 mm to 12.9 mm in patients aged 25-29 years. While patients aged ≥30 years can achieve the optimal clinical pregnancy rates (30-34 years old OR: 2.203, 95%CI: 1.387-3.501, P=0.001; ≥35 years old OR: 2.452, 95%CI: 1.524-3.945, P<0.001) and live birth rates (30-34 years old OR: 1.994, 95%CI: 1.260-3.155, P=0.003; ≥35 years old OR: 3.340, 95%CI: 1.975-5.648, P<0.001) when EMT≥13 mm. Conclusions The EMT on the day of hCG administration in each age group has significant correlation with the rates of clinical pregnancy and live birth. With the increase of EMT, clinical pregnancy rates and live birth rates are on the rise. It is recommended that patients aged 25 to 29 undergo fresh embryo transfer when EMT is ≥9 mm, and patients aged 30 or above undergo fresh embryo transfer when EMT is≥11 mm to obtain better pregnancy outcomes.
  • Study on the effect of diphthalate in follicular fluid on embryo quality in different ovarian response populations
    WANG Na, YANG Wenjing, LI Weiyu, WU Yang, QI Yuan, ZHANG Xuehong, SHEN Haofei, YU Xiao.
    Chinese Journal of Clinical Obstetrics and Gynecology. 2025, 26(4): 315-319. https://doi.org/10.13390/j.issn.1672-1861.2025.04.007
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    Objective To investigate the effect of di-2-ethylhexyl phthalate (DEHP) level in follicular fluid on embryo quality in different ovarian responders. Methods Prospective analysis of 164 patients who received assisted reproductive technology in the Reproductive Center of the First Hospital of Lanzhou University from January 2022 to June 2022. There were 80 cases with normal ovarian reserve (NOR), 39 patients with diminished ovarian reserve (DOR) and 45 patients with polycystic ovary syndrome (PCOS). The level of DEHP in follicular fluid of different ovarian responders was detected by ELISA. Multivariable Poisson regression analysis was performed to assess factors influencing the number of high-quality embryos and transplantable embryos, as well as DEHP exposure, across different ovarian response populations. Results Among the 164 patients, the concentration of DEHP in follicular fluid was (0.10±1.76) ng/ml in NOR group, (0.60±2.19) ng/ml in DOR group and (0.59±1.86) ng/ml in PCOS group. There was no significant difference in DEHP concentration among the three groups (P>0.05). Multivariate Poisson regression analysis showed that only the follicular fluid DEHP level in the NOR group had a significant effect on the number of high-quality embryos [(3.67±3.26)] [OR=0.902, P=0.002], and the other influencing factors had no significant correlation (P>0.05). Conclusions DEHP concentration had a significant effect on the number of high-quality embryos in the women with NOR. However, given the high levels of DEHP in follicular fluid observed in the DOR and PCOS patients, the prevention and control of the potential risk posed by this substance should be strengthened.
  • Effect of MTNR1B gene polymorphisms and basal body mass index interaction on susceptibility to gestational diabetes mellitus
    ZHANG Xirong, LIU Qian, LI Jianhua, WANG Weikai, LIU Xiaoling, WANG Yanxia.
    Chinese Journal of Clinical Obstetrics and Gynecology. 2025, 26(4): 320-324. https://doi.org/10.13390/j.issn.1672-1861.2025.04.008
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    Objective To investigate the effect of MTNR1B gene polymorphisms and basic body mass index (BMI) on susceptibility to gestational diabetes mellitus (GDM). Methods  From March to December 2021, 292 pregnant women diagnosed with GDM were randomly selected from Gansu Provincial Maternal and Child Health Hospital as the GDM group, with age as the matching condition. And 1:1 matching of healthy pregnant women with prenatal examination as the control group. The peripheral blood of the two groups was collected, and the genotype of single nucleotide polymorphism (SNP) at rs10830963 of melatonin recepters 1B (MTNR1B) gene was detected and analyzed by im-LDR technology, and the risk factors were screened by logistic regression, and a multiplicative model was employed to analyze the effects of the interaction between the rs10830963 SNP and basal BMI on GDM susceptibility. Results The basal BMI≥24.0 kg/m2 (OR=2.467, 95%CI: 1.684~3.615) was a risk factor for GDM, and carried GC (OR=1.484, 95%CI: 1.015~2.170) or GG (OR=1.840, 95%CI: 1.135~2.983) and allele G (OR=1.347, 95%CI: 0.069~1.697) and those with GC+GG dominant model (OR=1.572, 95%CI: 1.096~2.257) had the highest risk of GDM, and those with the GG genotype of rs10830963 of MTNR1B gene and a basal BMI of ≥24.0 kg/m2 had the highest risk of GDM (OR=3.504, 95%CI: 1.299~9.451). Conclusions The susceptibility to GDM in women with the MTNR1B rs10830963 GG and an underlying BMI of≥24.0 kg/m2 is greatly increased. Health education and bariatric prevention should be focused on this population to promote maternal and infant health outcomes.
  • Study on the correlation between vaginal laxity and vaginal microbiome in reproductive-aged women
    ZHAO Yuexin, LI Wenyan, WEI Xiaoting, WANG Shiyan, XIE Bing, SUN Xiuli.
    Chinese Journal of Clinical Obstetrics and Gynecology. 2025, 26(4): 325-329. https://doi.org/10.13390/j.issn.1672-1861.2025.04.009
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    Objective By investigating the compositional characteristics of vaginal microbiome in vaginal laxity patients and the distributional/functional differences compared with non-laxity populations, this study explores the correlation between vaginal microbiome and vaginal laxity, providing novel insights for its diagnosis and treatment. Methods A total of 30 vaginal laxity reproductive-aged patients (case group) and 30 non-laxity women (control group) were recruited from Peking University People's Hospital between May and November 2024. Vaginal secretion samples were collected, and bacterial 16S rDNA amplification technology was used to extract vaginal microbiome genomic DNA. Bioinformatics software was employed for data processing to compare the composition, distribution, and genetic function differences of vaginal microbiome between the two groups. Results The case group was predominantly composed of Prevotella, Atopobium, and Anaerococcus, while the control group showed Lactobacillus dominance with slightly higher Streptococcus abundance compared to cases. No significant α-diversity differences were observed (P>0.05), with highly overlapping Rank Abundance curves. β-diversity analysis demonstrated significant separation (Anosim R=0.117, P=0.01; Adonis R2=0.072, P=0.015). LEfSe-ROC integrated analysis identified biomarker genera: Peptoniphilus (AUC=0.74), Finegoldia (AUC=0.72), Prevotella (AUC=0.71), Porphyromonas (AUC=0.70), Atopobium (AUC=0.69), and Corynebacterium (AUC=0.69). Significant differential expression (P<0.05) was detected in multiple metabolic pathways and enzymatic synthesis gene pathways between groups. Conclusions Vaginal laxity in reproductive-aged women is correlated with vaginal microbiome composition.
  • serglycin promotes the growth of cervical cancer by regulating epithelial to mesenchy-mal transition
    JI Tingting, MA Jiale, QIN Yi, CUI Tianxia, WANG Xiaoyan, ZHAO Hong.
    Chinese Journal of Clinical Obstetrics and Gynecology. 2025, 26(4): 330-333. https://doi.org/10.13390/j.issn.1672-1861.2025.04.010
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    Objective To explore the correlation between the expression level of serglycin (SRGN) and epithelial-mesenchymal transition (EMT) in cervical cancer. Methods The expression level of SRGN in cervical cancer was predicted using the GEO database. Pathological sections from 70 patients with cervical cancer were collected, and the expression of SRGN protein in cervical cancer tissues was detected by immunohistochemical two-step method and direct immunofluorescence. The effects of SRGN on the migration and EMT of Hela cells were detected by transfection technology, scratch test and Western blotting. Results GEO data analysis showed that the expression of SRGN in cervical cancer tissues was significantly higher than that in normal tissues (P<0.05), and the difference was statistically significant. Pathological feature analysis showed that SRGN was closely related to pelvic or para-aortic lymph node metastasis and TNM stage (StageⅢ,Ⅳ) (P<0.001). In vitro cell experiments indicated that silencing SRGN inhibited the migration and EMT ability of Hela cells (P<0.01). Conclusions SRGN promotes the growth of cervical cancer cells by influencing the progression of EMT.
  • Effects of estradiol valerate on Th1/Th2 cells in BALB/c mice after hysterectomy
    HUANG Hui, YI Hongru, ZHONG Wenhua, CHANG Penghuan, YANG Zhaoxin.
    Chinese Journal of Clinical Obstetrics and Gynecology. 2025, 26(4): 334-338. https://doi.org/10.13390/j.issn.1672-1861.2025.04.011
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    Objective To investigate the effect of estradiol valerate on Th1/Th2 cells in BALB/c mice after hysterectomy. Methods The mice were randomly divided into blank control group, model group and treatment group, the mice in the treatment group were given estradiol valerate by gavage on the 6th day after operation, while the blank control group and the model group were given equal volume of sterile water for injection, once a day for 15 days; Keyhole limpet hemocyanin (KLH) was injected subcutaneously on the 5th and 12th day after treatment; The changes of lymphocyte subsets(CD4+, CD8+) and Th1,Th2 cells were detected by flow cytometry; T-cell dependant antibody response test (TDAR) was performed using immune microsphere technology. Results Compared with the control group, the ratio of CD4+ and CD8+ subpopulations, antibody IgG content, and the levels of Th1 and Th2 decreased in the model group after surgery (P<0.05). The Th1/Th2 ratio increased slightly, but the difference was not statistically significant (P>0.05). After the administration of estradiolvalerate, the above indexes were restored to different degrees, the proportions of CD4+ and CD8+ subsets, the content of antibody IgG, and the levels of Th1 and Th2 cells were significantly increased (compared with the model group, P<0.05); however, the Th1/Th2 ratio was reduced compared with the model group (P<0.01), inducing the body's immune function to be biased in the direction of humoral immunity. Conclusions Estradiol valerate can improve humoral immunity in mice after total hysterectomy by regulating the differentiation of Th1 and Th2 cells.
ISSN 1672-1861　CN 11-4967/R
Responsible Institution
Ministry of Education People's Republic of China
Editing
Editorial Board of CCOG
Editor in chief
WEI Lihui
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